In vitro and in vivo Antibacterial Studies of Medicinal Plant on Motile Aeromonad Septicemia in Fish Caused by Aeromonas hydrophila

摘要

Aeromonas hydrophila , gram negative facultative anaerobic short bacillus, causes red fin disease, haemorrhagic septicemia, motile aeromonad septicemia and other infections in Carassius auratus . Multiple drug resistance of some strains of A. hydrophila presents a major threat in the control these diseases. Recently much attention has been paid to extracts and biologically active compounds isolated from plant species. In this studies ten plants leaves were assayed against Aeromonas hydrophila both in vitro and in vivo . The effective plant was found to be Phyllanthus emblica which shows the 24 mm zone diameter which was the nearest to oxytetracyclin and further choose for the in vivo study. Tagetes erect , Azadirachta indica , Calotropis procera also showed nearer activity nearer to the control antibiotic. There were no effect of Momordica charantia , Achyranthes aspera , Centella asiatica on aeromonads disease as the solvent alcohol solely gave the zone diameter of 14 mm. When incorporated P. emblica leaf extract in infected gold fish at a dose of 25 mg g-1 of body weight, the A. hydrophila infection was cured within 12-15 days under laboratory condition.