Analytical and Biological Evaluation of Two Schiff?￠????s Bases: Spectrophotometeric Analysis of Copper (II) in Water and Soil Samples

摘要

Two novel ligands, (E)-N1-(2-hydroxy-5-nitrobenzlidene) isonicotinoylhydrazone (HNBISNH)/2-(4-fluoro benzlideneamino)benzenethiol (FBBT) were synthesized and used for the quantification of copper (II) in various water and c HNBISNH/FBBT interacts with copper (II) to form 1:1 [L:M] orange /brick red color complexes in presence of phosphate buffer of pH 3.7/4.5 which increases the sensitivity of the complexes. Thus formed colored complex followed the Beer’s law up to 1.6 and 1.9 mg La??1 with an optimum concentration range over 0.11-1.3 mg L-1 and 0.10-1.7 mg L-1, the slope of Ringbom’s plot are found to be 0.3500 and 0.3300 for copper(II)-HNBISNH and copper(II)-FBBT complexes, respectively. The molar absorptivity and Sandell’s sensitivity of copper (II)-HNBISNH and copper(II)-FBBT complexes were calculated and found to be 4.91×104 L mol?1 cm?1, 6.1×104 L mol?1 and 0.0010 μg cm?2 and 0.0014 μg cm?2 respectively. The detection limits was calculated and found to be 0.043 μg L?1 and 0.036 μg L?1 with HNBISNH and FBBT respectively. Various optimum conditions such as effect of pH, reagent concentration, accuracy, precision and reproducibility were investigated to improve the sensitive for the present method. The detailed study of various excipients confirmed the high selectivity of the method. The proposed method was applied to the determination of copper (II) in different water & soil samples obtained results were in good agreement with atomic absorption spectrometric method (AAS).