Response of Salivary Antioxidants to Intense Exercise in Non-Athlete Men

摘要

Nazari Y, Damirchi A, Sariri R, Taheri M. Response of Salivary Antioxidants to Intense Exercise in Non-Athletic Men. JEPonline 2012;15(3):1-9. Both enzymatic and non-enzymatic antioxidants in human saliva were subjected to alteration due to different factors. The purpose of this study was to determine the effects of a short period intense exercise on salivary flow rate, superoxide dismutase (SOD) activity, and uric acid concentration. Using a double-blind randomized design, 25 healthy non-athlete men (mean ± SD = 21.34 ± 3.11 yrs) volunteered to participate in the study. The purpose of the study was explained and, then, an informed consent was completed prior to treadmill runs at exhaustion. Their unstimulated whole saliva samples were collected before, immediately after, and 1 hr after exercise. Activity of superoxide dismutase was determined nitro-blue tetrazolium (NBT) as its specific substrate. The enzymatic assay was based on the ability of superoxide dismutase to inhibit the reduction of NBT by superoxide. The results showed a significant increase in uric acid and SOD activity immediately after exercise, which almost returned to the original level in 1 hr. It was concluded that an exercise of short-duration, high-intensity increases the antioxidant power of saliva, while only small changes are observed in saliva flow rate.