Preliminary Study to Investigate the Optimum Parameters of using Hematoporphyrin IX to Control Flesh Fly ( Parasarcophaga argyrostoma )

摘要

Gastric, intestinal and nasal myiasis are animal diseases caused by flesh fly ( Parasarcophaga argyrostoma ). This study aims to cut the cycle of flesh fly at the adult stage to reduce the animal infection and control these diseases using sunlight and photosensitizer (Hematoporphyrin IX). This class of compounds is environmentally friendly with a high photosensitizing activity towards biological systems. It is approved for medical use in the photodynamic therapy of tumors and other diseases. Hematoporphyrin IX becomes toxic only when it is activated by sunlight. Parasarcophaga argyrostoma is readily attracted by sugar bait containing hematoporphyrin amounts in the small range (treated flies). Moreover, it appears to consume enough of the bait to allow the Hematoporphyrin IX (HP) to exert its phototoxic action when the insect is exposed to direct sunlight. The treated and untreated flies were dissected for midgut histological studies. The results of testing hematoporphyrin IX (HP) as a photoinsecticide is presented. 10-2 M L-1 HP caused the highest mortality (83%) and 1935 W m-2 sunlight intensity was enough to cause 96% mortality of HP treated flies. The results revealed that HP appears to be very active against Parasarcophaga argyrostoma . HP showed high ability to accumulate inside the insect organs The histological studies of P. argyrostoma alimentary canal showed high extent of alimentary canal tissue damage as a function of sunlight exposure times after incubation with 10-2 M L-1 HP concentaration. This study concluded the highest efficiency of HP to induce reduction of Parasarcophaga argyrostoma population, which can be used as a novel modality of its control.