Effect of Sublethal Dose of Hexaflumuron on the Ovary and Egg Maturation of Spiny Bollworm, Earias insulana (Boisd.)

摘要

The effect of LC20 of hexaflumuron on some biological aspects, egg maturation and ovary of the spiny bollworm, Earias insulana (Boisd.) were studied under controlled conditions of 26±1°C and 75±5 RH. The calculated LC20 and LC50 values of hexaflumuron were 17.42 and 72.75 ppm. The treatment newly hatched larvae with LC20 (17.42 ppm) elongated larval and pupal duration compared with control. Treatment significantly reduced mean numbers of deposited eggs and hatchability percentage compared with control. Hexaflumuron reduced number of immature eggs after emergence (20.56 eggs/ovariol) compared with 27.05 eggs/ovariol for untreated. No mature eggs were present in the female moths ovarioles after emergence of treated and untreated. Mature eggs were found after 36 h and 3 days in untreated and treated, respectively. The highest numbers of mature eggs 3.63 and 6.32 eggs/ovariol were recorded after four days from emergence in treated and untreated, respectively. Hexaflumuron reduced the ovarian weight and length, size of basal eggs and number of spermatophore/female compared with untreated. The LC20 of hexaflumuron reduced significantly larval content of total carbohydrate, lipid and soluble protein.