Phytochemical screening, antioxidant activity and estimation of quercetin by HPLC from Caesalpinia bonducella

摘要

Caesalpinia bonducella is an important medicinal plant for its traditional uses against different types of diseases. The aim of the present study was to evaluate in vitro antioxidant activities, phytochemical analysis and HPLC analysis of ethanol extract of leaves of Caesalpinia bonducella collected from Bhopal region of Madhya Pradesh. Qualitative analysis of various phytochemical constituents and quantitative analysis of total phenolics flavonoids and alkaloids were determined by the well-known test protocol available in the literature. The in vitro antioxidant activity of ethanolic extract of the leaves was assessed against DPPH and Nitric oxide scavenging activity using standard protocols. Quercetin was detected in ethanolic extract of Caesalpinia bonducella under study by using RP-HPLC analysis. Phytochemical analysis revealed the presence of phenols, flavonoids, tannins, saponins, alkaloids. The total phenolic, flavonoids and alkaloids content of ethanolic leave extract of Caesalpinia bonducella was found to be 0.647, 0.941and 0.369mg/100mg respectively. Caesalpinia bonducella extracts showed effective DPPH radical scavenging and nitric oxide scavenging activity. Quercetin content was found in Caesalpinia bonducella extract, was quantified as 0.285μg/ml. HPLC analysis of plant extracts indicated the presence of the most abundant dietary flavonol, Quercetin. The results of this study revealed that the bioactive compound content differences could be determinant for the medicinal properties of this plant especially for antioxidant activities with potential applications in food and pharmaceutical industries.