Altered expression of circular RNAs in human placental chorionic plate‐derived mesenchymal stem cells pretreated with hypoxia

摘要

Background Hypoxic preconditioning alters the biological properties of mesenchymal stem cells (MSCs). It is not known whether this process has an effect on circular RNAs (circRNAs) in MSCs. Methods Human placental chorionic plate‐derived MSCs (hpcpMSCs) isolated from the same placentae were classed into two groups: hypoxic pretreated (hypoxia) group and normally cultured (normoxia) group. The comparative circRNA microarray analysis was used to determine circRNAs expression and verified by quantitative reverse‐transcription polymerase chain reaction (qRT‐PCR) in the two groups. Results One hundred and two differentially expressed circRNAs in the hypoxia group were found compared to that in the normoxia group (fold change 1.5‐fold and P Conclusions The results indicate that the altered expression of specific circRNAs in MSCs is associated with hypoxic preconditioning. This finding provides further exploration of underlying mechanisms of the characteristic changes of MSCs with hypoxic preconditioning.