Molecular Detection of Hemoplasma Species (Mycoplasma spp.) in Cattle in Cebu, Philippines

摘要

Epidemiological reports on vector-borne diseases affecting cattle in the Philippines have been limited. The present study aimed to detect the presence of the Hemoplasma pathogen (Mycoplasma spp.) in dispersal cattle in selected plainlands and marginal uplands in Cebu, Philippines, using peripheral blood smear examination (PBSE) and polymerase chain reaction (PCR) methods. A total of 14 cattle were tested. Using PBSE, no inclusion bodies were observed. However, using PCR, 4 out of 14 cattle showed positive results. Results indicate the high sensitivity of the PCR methods in detecting Mycoplasma spp. than that of the PBSE. The present study adds new information on the biodiversity of vector-borne pathogens in cattle in Cebu, Philippines, and is the first report of detection in the country.