A Simple Automated Method for the Determination of Nitrate and Nitrite in Infant Formula and Milk Powder Using Sequential Injection Analysis

摘要

A fast and efficient automated method using a sequential injection analysis (SIA) system, based on the Griess, reaction was developed for the determination of nitrate and nitrite in infant formulas and milk powder. The system enables to mix a measured amount of sample (previously constituted in the liquid form and deproteinized) with the chromogenic reagent to produce a colored substance whose absorbance was recorded. For nitrate determination, an on-line prereduction step was added by passing the sample through a Cd minicolumn. The system was controlled from a PC by means of a user-friendly program. Figures of merit include linearity ( r ~(2) > 0.999 for both analytes), limits of detection (0.32?mg kg~(?1) NO_(3)-N, and 0.05?mg kg~(?1) NO_(2)-N), and precision (s_(r)%) 0.8–3.0. Results were statistically in good agreement with those obtained with the reference ISO-IDF method. The sampling frequency was 30 hour~(?1) (nitrate) and 80 hour~(?1) (nitrite) when performed separately.