In-Cell NMR Spectroscopy?In vivo Monitoring of the Structure, Dynamics, Folding, and Interactions of Proteins at Atomic Resolution

摘要

Multidimensional nuclear magnetic resonance spectroscopy (NMR) is a very versatile technique which is routinely used for the characterization of 3D structure, backbone dynamics, and intermolecular interactions of proteins in solution at atomic resolution.NMR spectroscopy as an analytical technique has come of age. Availability of higher field spectrometers, equipped with cryoprobes, has drastically increased the sensitivity and resolution of NMR data. In recent years, several new pulse sequences have been developed which have significantly diminished the perceived molecular-size limitation of NMR spectroscopy. Currently, determination of 3D solution structures of proteins, with molecular mass in the 100 kDa range, is no longer impossible.