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Adult Rat Heart Cell CultureThe Morphological Study of Tissue Fragments on Various Environments in Culture

机译:成年大鼠心脏细胞培养在不同培养环境中组织碎片的形态学研究

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This paper reports a modification of Jacobson's method1), 2) for culturing adult rat heart cells after mechanical and enzyme treatment. For heart cell dissociation, it is useful to wash tissue fragments 3 times with saline A solution after enzyme treatment, and it is important to change the medium immediately after culturing heart cells in order to prevent myocardial cells from degeneration caused by the enzyme solution and toxins in the cell debris.
机译:本文报道了一种改良的Jacobson方法1),2),用于在机械和酶处理后培养成年大鼠心脏细胞。对于心脏细胞的解离,酶处理后用盐水A溶液清洗组织碎片3次非常有用,并且在培养心脏细胞后立即更换培养基对于防止心肌细胞因酶溶液和毒素引起的变性很重要。在细胞碎片中。

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