In vitro Optimization of White Yak (Bos grunniens) Oviduct Epithelial Cells Culture

摘要

Epithelial cells of the mammalian oviduct play an important role in reproductive and developmental events. Therefore, the objectives of the present study was to establish a method for isolation and culture of White yak (Bos grunniens) oviduct epithelial cells in vitro and to determine the effect of Fetal Calf Serum (FCS), Epidermal Growth Factor (EGF) and Transforming Growth Factor-β1 (TGF-β1) and their combination on White yak oviduct epithelial cells. The results showed and 100 ng mL-1 of EGF in TCM 199 with 10% FCS could significantly increased cell numbers of yak oviduct epithelial. Alone no addition, EGF (10 ng mL-1) and TGF-β1 (1 ng mL-1) had no effect on cell number. However, when included with 10% FCS in medium yak oviduct epithelial cell numbers significantly increased on day 6 of culture. The combination of FCS (10%) and EGF (10 ng mL-1) is the ideal formula to culture yak oviduct epithelial cells. Co-culture with yak oviduct epithelial cells the developmental rate of blastocyst was 8.9%. The results support the culture environment that oviduct epithelial cell may have direct effects on yak embryo developmental block in vitro production.