Cloning and Sequence Analysis of Donkey Growth Hormone Gene

摘要

The PCR primers were designed online according to result of gene homology comparison. Donkey GH gene DNA and cDNA sequence were cloned from liver and blood by RT-PCR and PCR, compared with GH gene sequences of different species by bioinformatics. The donkey GH gene sequence was 1928 bp including the 706 bp cDNA sequence with the complete CDS. By comparison for DNA and cDNA sequences, it was found that the GH gene sequence included 5 exons and 4 introns, encoding 216aa including signal protein of 26aa and matured protein of 190aa. Based on the analysis of the similarity of GH genes in different species on the level of cDNA, DNA and the deduced amino acid there was the most homology to the horse. The GH gene of donkey was conservative in the process of evolution and its promotor was not specific TATA box but was CATA box. The mutation of C→G in 1267 may affect the growth and development of donkeys and horses. All researches made an essential foundation for GH regulation of gene expression, evolution, polymorphism analysis in the future.