Antibacterial Comparative Study Between Extracts of Mexican Propolis and of Three Plants Which Use Apis mellifera for its Production

摘要

The aim of this study was to evaluate the antibacterial properties of extracts obtained from three Mexican plants (Callistemon citrinus Stapf, Eucalyptus camaldulensis Dehnhardt and Ricinus communis L) and of extracts of propolis produced by Apis mellifera in the same region comparing them by diffusion and plate dilution tests. Hexane and ethyl and methanol acetate extracts from each plant as well as propolis hexane and ethanol extracts were tested on 2 gram-positive and 8 gram-negative bacterial strains: Staphylococcus aureus, S. epidermidis, Shigella dysenteriae, Salmonella tiphy, Yersinia enterocolitica, Enterobacter aerogenes, Enterobacter agglomerans (Pantoea agglomerans), Escherichia coli, Vibrio cholerae Nr. 01 and V. cholerae (clinical case). The propolis extracts was analyzed by HPLC chromatography. Propolis extracts were active against Staphylococcus aureus, S. epidermidis, Vibrio cholerae Nr. 01 and V. cholerae (clinical case). Callistemon citrinus Stapf extracts were effective against Staphylococcus aureus, S. epidermidis, Vibrio cholerae Nr. 01 and V. cholerae (clinical case), Eucalyptus camaldulensis Dehnhardt extracts acted against Staphylococcus aureus, S. epidermidis and Vibrio cholerae Nr. 01 and Ricinus communis L extracts were effective against V. cholerae (clinical case), Shigella dysenteriae and E. agglomerans. In general, antimicrobial activity of propolis coincided with plant extract activity against Staphylococcus aureus, S. epidermidis, Vibrio cholerae Nr. 01 and V. cholerae (clinical case). The major compounds were the flavones, cinnamic acid derivative and the caffeic acid derivative. These data corroborate the close relationship between propolis composition and the constitution of plants serving as source for some of its components. Thus, the antibacterial spectrum of propolis will vary depending on the geographical situation and the types of vegetation of a given region. On the other hand, plants used by Apis mellifera to produce propolis are being considered as source of secondary metabolites with antimicrobial action.