The Genetic Diversity of Hainan Black Goat by Capillary Gel Electrophoresis with Microsatellite Markers in Tropical Area of China

摘要

The genetic diversity and relationships of Hainan Black goat breed among 8 populations were studied using 14 microsatellite markers. In the 14 microsatellite loci, the number of alleles per locus ranged from 15 (BMS1248) to 44 (BMS2258) and the Ho values ranged from 0.003 (BM6404) to 0.923 (BM6444) among the 8 groups, the mean Polymorphic Information Content (PIC) of all the primers detected for all groups showed high values (0.892). For all the goat groups, the mean number of alleles ranged from 9.577 (Baisha) to 16.571 (Saya), the average observed heterozygotes, expected heterozygosity, Polymorphism Information Content (PIC), allelic richness and effective number of alleles was 0.402, 0.866, 0.838, 7.625 and 12.425, respectively. Most of the 14 loci were in Hardy-Weinberg equilibrium (p>0.05) except locus BMS2258 (p<0.0001) and all the 8 populations were in Hardy-Weinberg equilibrium (p>0.05) too. The percentage of variation among the 8 goat groups only amounted to 5.11%, the remaining existed among individuals within populations (58.12%) and within individuals (36.77%). The genetic differentiation pattern and genetic relationships among the 8 populations displayed some degree of consistency with their geographical locations expect Sanya and Linshui regions and suggested that exist a certain inbreeding between SY and CM. The Fis indicated some degree inbreeding existed in Changjiang goat group and there was no significant among the 8 populations. This study will help to interpret the genetic characters of Hainan Black goat and benefit to the future conservation programs.