The aim of this study was to evaluate and compare adjuvant potential of two widely used Chinese medicine prescription preparations Golden Screen health Capsules (GSC) and Yupingfeng Koufuye (YPF) on immune responses to Ovalbumin (OVA) in mice. Mice were administered orally with GSC or YPF at the different doses for 5 days and then immunized subcutaneously twice with OVA 100 μg alone at 2 weeks intervals. The 2 weeks later, splenocyte proliferation, Natural Killer (NK) cell activity, production of cytokines from splenocytes and serum OVA-specific antibody titers were measured. GSC and YPF significantly not only the serum OVA-specific IgG and IgG1 antibody titers but remarkably enhanced Con A-LPS and OVA-induced splenocyte proliferation and NK cells activities in OVA-immunized mice. The production of cytokine IL-2 from splenocytes and serum OVA-specific IgG2a and IgG2b antibody titers in the immunized mice were also significantly enhanced by YPF. YPF was better than GSC and alum in the quality and strengths to enhance specific cellular and humoral immune responses against OVA in mice with YPF preferred for those situations where mixed Th1/Th2 responses are needed.
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