Changes in Blood Gas Composition and Acid-Base Equilibriums in Cattle Blood Samples Kept under Different Temperature Regimens and Times

摘要

Changes in venous blood gas composition and acid-base equilibrium in Holstein cattle blood samples kept under different temperatures at various time points were investigated for 24 h. The blood samples were collected from 3 healthy multiparous Holstein cows. A total of 9 blood samples obtained from the animals were allocated into three groups and kept at +4°C for group 1 (n = 3), at room temperature, 22°C, for group 2 (n = 3) and in an incubator at 37°C for group 3 (n = 3). Blood gases were analyzed at 0, 1, 3, 6, 12 and 24 h after the storage. The analyses of the blood samples indicated no change at pH and pCO2 at 4 and 22°C for the 1st h. Similarly, in addition to O2SAT at 4°C for 3 h and at 22°C for 6 h; O2CT at 4°C and at 22°C for 24 h, HCO3 and BB values at 4, 22 and 37°C for 24 h remained unchanged. By contrast, PO2, BE and glucose levels in the samples were markedly altered within the 1st h.