Antifungal Susceptibility of Dermatophytes Isolated from Domestic Calves in Thamar, Yemen

摘要

This research aimed to isolate and identify some dermatophytes which causing ringworm disease in calves in Thamar government, Yemen Republic and evaluate antifungal susceptibility of isolates against some common chemical compounds (CuSO4+CaO, Formaldehyde and Hydrogen Peroxide and Amphotericin B was used as standard antifungal agent). A total of 139 samples were collected from head, chest and limbs of calves which infected by ringworm disease. Dermatophytes Test Medium (DTM) was used to differ between dermatophyte and non dermatophyte fungi. Only 11 isolates were considered dermatophytes. Macro and microcharacteristics were studied to identify the dermatophyte isolates. All isolates were identified as Trichophytonverrucosum. The identification of the isolates were confirmed by Assiut University Mycological Center (AUMC), Egypt and were given AUMC NO. 2407. In vitro antifungal susceptibility test was performed according to Committee for Clinical Laboratory Standards for filamentous fungi (NCCLS M38-A) guidelines. The study concluded that T. verrucosum could be major causative agent of ringworm disease in calves in Thamar, Yemen. Of all the farms, the farms of government sector had the highest ringworm disease prevalence rate. In vitro activity of Formaldehyde solution (10%) against T. verrucosum isolated from calves was more than mixture of cupric sulphate with calcium oxide and hydrogen peroxide. It could be used as antifungal drugs to treat ringworm disease in calves in Yemen.