The Effect of Helicobacter pylori on Apoptosis of the GES-1 Transfected pEGFP-N1-UreB Eukaryotic Expression Plasmid in vitro

摘要

To investigate the effect of Helicobacter pylori on apoptosis of the GES-1 transfected pEGFP-N1-UreB eukaryotic expression plasmid in vitro. The Hp-UreB gene was amplified by PCR and cloned into eukaryotic expression plasmid pEGFP-NI to construct recombinant plasmid pEGFP-NI-UreB and transfected into GES-1 cells and screened by G418. GES-1 cells transfected into plasmid pEGFP-NI-UreB were co-cultured with H. pylori SS1 and its apoptosis was quantified by flow cytometry using Annexin V-APC and 7-AAD. Changes in cell morphology were observed by transmission electron microscope. The recombinant pEGFP-N1-UreB was constructed and stable transfected into GES-1 cells. The apoptotic rates of cells transfected into pEGFP-N1-UreB plasmids were significantly lower than those of control cells (p<0.05).