Optimization of root proliferation medium for Hyoscyamus niger L.

摘要

Untransformed root culture ofHyoscyamus nigercould be induced from petiole explants on solid MS medium supplemented with 2.0 mg l–1IBA after four weeks of incubation under continuous light of 32.5 µmol m-2s-1. However, liquid MS medium supplemented with 0.5 mg l–1IBA was found to be better for root proliferation. Cultures at the third subculture cycle during root proliferation showed maximum root biomass production either under continuous light (3.69±1.00 g) or in total darkness (4.02±0.94 g). Step-wise optimization of culture medium components was carried out by manipulating the MS macronutrients, myo-inositol, Fe-EDTA and sucrose. The optimum root biomass producing medium was established as modified MS medium containing altered concentrations of macronutrients (15.0 mM NH4NO3, 33.1 mM KNO3, 3.19 mM CaCl.2H2O, 1.9 mM MgSO4.7H2O and 1.4 mM KH2PO4), 9.6 mM myo-inositol, 0.1 mM Fe-EDTA and 3% sucrose supplemented with 0.5 l–1mg IBA.