Strain-Dependent Migration of CD4 and CD8 Lymphocyte Subsets to Lymph Nodes in NOD (Nonobese Diabetic) and Control Mice

摘要

Subpopulations of lymphoid cells were compared with respect to their ability to migrate intoperipheral lymphoid organs of nonobese diabetic (NOD) mice and various strains of controlmice. In short-term,in vivohoming studies, no major differences in the pattern of homingof B and T cells were observed among all mouse strains studied. On the other hand, CD4cells localized consistently more efficiently than CD8 cells in both PP and LN of adult NODand BALB/c mice, whereas both populations migrated roughly equivalently in LN of adultDBA/2, CBA, and C57BL/6 mice. No age-dependent differences in the homing of CD4 andCD8 cells were observed in BALB/c mice. On the contrary, in 2-week-old NOD mice, CD4and CD8 cells migrated equally well. The preferential entry of CD4 cells in adult NOD andBALB/c did not result from increased blood transit time of CD8 cells. On the other hand,the preferential migration of CD8 cells was observed in the liver, whereas the two T-cellsubsets migrated equally well in the lungs. The differences in the homing characteristics ofCD4 and CD8 cells among NOD, BALB/c, and C57BL/6 mice were not related tomodifications in the level of expression of adhesion molecules such as MEL-14, LFA-1, andPgp-1.