Formulation, HPTLC Method Development and Validation of Gallic Acid in Health Drinks

摘要

The present research work supports formulation, development of health drinks containing Withania somnifera, Ocimum sanctum, Glycyrrhiza glabra, Emblica officinalis, Asparagus racemosus, Tribulus terrestris, Terminalia arjuna, Tinospora cordifolia, Chlorophytum borivilianum, Piper nigrum, Curcuma longa, Myristica fragrans as an active ingredients. The product was developed by treating concentrates of each crude drug with purified water. A new, simple, sensitive, selective, precise and robust high-performance thin-layer chromatographic (HPTLC) method for analysis of Gallic acid from health drink was developed and validated. Pre-coated silica aluminium plate 60F-254 (20 × 10 cm) with 200 μm thickness was used as stationary phase while toluene: ethyl acetate: methanol: formic acid (3: 3: 0.2: 0.8 v/v) system was developed as a mobile phase. Spectrum analysis showed the same Rf values and spectrum pattern of standard and sample. The developed method was quite good and most sensitive for the present products.