The diversity of bacteria in blood cultures of susp ected patients with typhoid fever based on fenetics numerical analysis and resp onse to antibiotics in Jayapura, Papua

摘要

Suspected patients with typhoid fever is diagnosed patients and physical examination found symptoms of fever, gastrointestinal disorders and disorders of conscio usness with clinical syndromes of typhoid incomplet e so it needs to be supported by laboratory description that show s typhoid, including bacteriological examination of blood cultures and serologic widal. Bacteremia-septicemia is a complication that causes typhoid fever bacter ia in the blood. Research using laboratory experimental metho d is implemented in health laboratory of Jayapura, Papua Province, with reference checks Test Widal obtained 135 patients in the city of Jayapura. Widal Test p ositive in 88 samples (65.2%) were found 81 samples (92.0%) patie nts with suspected typhoid fever has no bacterial g rowth (sterile), 7 samples (8.0%) grew gram-positive cocc i (57.1%) and bacterial Gram-negative bacilli (42.9 %). Based on fenetics numerical analysis using the Vitek 2 co mpact and test of sensitivity to 14 kinds of antibi otics, bacteria are obtained diversity of Gram-negative bacilli as much as 3 isolates; Escherecia coli, Pseodomonas ae ruginosa, Pseudomonas maltophila and 4 isolates of gram-posit ive cocci such as Staphylococcus aureus, Staphyloco ccus epidermidis, Staphylococcus hominis and Staphylococ cus saprophyticus. Dendogram of fenetics structure numerical analysis results, obtained 6 clusters with similari ty between 56.6% - 97.0% use reference strains of S . aureus subspecies aureus ATCC 25923 and P. aeruginosa ATCC 27853. Sensitivity of 7 isolates were found agains t 14 types of antibiotics obtained Nitrofurantoin and Ge ntamycin have high sensitivity (100%), while Trimet hoprim - Sulfamethoxazole has decreased sensitivity of 57.1% and 92.3% (12/13) of sensitive antibiotics against Gram positive cocci and 63.6% (7/11) of antibiotic-resis tant bacteria Gram-negative bacilli.