Evaluation of anti-inflammatory, analgesic and immunosuppressant activitiesof aceclofenac solid dispersions on wistar rats.

摘要

The present research work was focussed on the preparation as well as the evaluation of Aceclofenac solid dispersions for analgesic, anti-inflammatory and immunosuppressant activities. Solid dispersions were formulated using cros-carmellose sodium as polymer in different ratios. The solid dispersions were prepared using physical mixture, solvent evaporation and kneading techniques. The solid dispersions were found to release the drug faster than the pure drug in dissolution media of phosphate buffer pH 7.0. The formulation ACK4prepared by kneading technique, showed best drug release profile when compared to other formulations. This formulation was used for the evaluation of various pharmacological activities on Wistar rats. Heat induced haemolysis and protein denaturation methods were selected for the evaluation of Ex-vivo anti-inflammatory activity using fresh rat blood. Whereas, the traditional hot plate method is used for evaluation of analgesic action. A novel technique was employed for evaluation of immunosuppression activity using fungi. When compared to the control group, the group of animals treated with ACK4 formulation showed better results. From the test results, a reduction in haemolysis was observed. It also showed reduction in pain perception and immunosuppression. Thus from the present study it was concluded that Aceclofenac solid dispersion could be a promising analgesic, anti-inflammatory and immunosuppressant agent which would be a boon in organ-transplantation and cardiac patients.