Spectral characterization, docking and in-vivo anti-inflammatoryactivity of Isoivangustin, a constituent isolated from methanolextract of Cyathocline purpurea

摘要

A sesquiterpene lactone, isoivangustin was isolated from the whole plant Cyathocline purpurea (Buch-Ham ex D. Don.) Kuntze (Fam. Asteraceae). The structure of this compound was elucidated and established by standard spectroscopic methods (IR, 1H-NMR, 13C-NMR, DEPT and MS data). Carrageenan induced paw edema model was used for evaluation of anti-inflammatory activity. Isoivangustin was subjected to molecular docking study to find out the binding interactions with the active site of TNF-alpha converting enzyme (TACE). Isoivangustin was found to be active in reducing inflammation (29.00 %) which was comparable to diclofenac (34.57 %) at 3rd hr. The docking score of isoivangustin and diclofenac with TACE were -5.341 and -7.358, respectively. In conclusion the result suggested that isoivangustin, a sesquiterpene lactone isolated from Cyathocline purpurea showed good antiinflammatory activity which may be mediated by inhibition of tumor necrosis factor (TNF)-α.