Development of uv spectrophotometric vierodta??s method for thesimultaneous estimation of abacavir and lamivudine in combinedtablet dosage form

摘要

Simple, accurate, precise, economical and reproducible analytical method have been developed for the simultaneous estimation of abacavir and lamivudine in pure bulk drug and in combined tablet dosage form by UV spectrophotometric Vierodt’s method. The stock solutions were prepared in mixture of acetonitrile and methanol (3:2) followed by the further required dilutions with distilled water. In proposed Vierodt’s method, the lmax for the estimation of abacavir and lamivudine were selected at 260 nm and 271 nm respectively. Linearity in concentration range of 5-25 mg/ ml by both the drugs was found. The method has estimated abacavir 100.01%, lamivudine 100.02% in the bulk drug and 99.86%, 99.82% of the both in tablets respectively. The results of analysis have been validated statistically and also by recovery studies. Thus the present study gives excellent method for the determination of both the drugs in combined tablet formulation.