Innovative synthesis and anti-microbial activity study of innovative Mannich bases containing 2-phenoxy-1,3,2-dioxa phospholanes and indole systems

摘要

In the present study, synthesis, characterization, antimicrobial activity on some novel pyrazolone derivatives has been taken up. Reaction of (4Z)-2-{4-[(15Z)-4-(2-phenylhydrazono)-4,5-dihydro-3-methyl-5-oxopyrazol-1-yl] phenylamino}-N'-(2-oxoindolin-3-ylidene) acetohydrazide and acetophenones were refluxed in methanol containing a catalytic amount of glacial acetic acid for 4 hours furnished the corresponding (19Z)-2-{4-[(4E)-4-(2-substituted aryl hydrazono) -4,5 -dihydro-3 –methyl -5-oxopyrazol -1-yl] phenylamino}-N'-(1-phenylethylidene) acetohydrazide step-1 product in excellent yields. Step-I products and excessive acetic anhydride was refluxed to afforded (4E)-4- (2-substituted aryl hydrazono)-1-{4-[(4-acetyl-4,5-dihydro-5-methyl-5-phenyl-1,3,4-oxadiazol-2-yl) aminomethyl] phenyl}-3-methyl-1H-pyrazol-5(4H)-one step-II product. The structural assignments to compounds Step-I product and Step-II product were based on their elemental analysis and spectral (IR, 1HNMR and MS) data. The antibacterial activity of synthesized compound was studied by the disc diffusion method against pathogenic bacteria and fungi.