New development of carbonate apatite-chitosan scaffold based on lyophilization technique for bone tissue engineering

摘要

Carbonate apatite-chitosan scaffolds (CA-ChSs) were fabricated using the lyophilization technique. It was found that ChSs prepared with 200 mg chitosan powder (ChSs_(200)) had well-structured three-dimensional architecture with high porosity and good retentive form without brittleness. In addition, it was shown that the number of osteoblast-like cells MC3T3-E1 proliferated on desalinated ChSs_(200) was larger than that on the non-desalinated ChSs_(200). CA-ChSs were fabricated by adding 100 mg carbonate apatite (CA) to 200 mg chitosan gels followed by freeze-drying (CA_(100)ChSs_(200)). SEM observation revealed that CA_(100)ChSs_(200) had favorable three- dimensional porous structures. The number of living cells increased more rapidly on CA_(100)ChSs_(200) prepared with different amounts of CA than on ChSs. ALP activity significantly increased after day 14 and reached a plateau after day 21 in ChSs_(200) and CA_(100)ChSs_(200). It was concluded that newly developed CA_(100)ChSs_(200) may be a possible scaffold material for tissue engineering.