Study of Okra Powder (Abelmoscus Esculentus) Effects on Histology of Liver Tissue and Sero-Biochemical Parameters in Diabetic Rats (HFD/STZ)

摘要

Introduction: Type 2 diabete is a kind of metabolic disease that it is associated with hyperglycemia, hyperlipidemia and disturbed liver function.  The aim of the present study was to evaluate the protective effects of Okra Powder on liver damage in high fat diet fed / streptozotocin (HFD-STZ)-induced type 2 diabetic rats.Methods: In this experimental study, 25 Wistar Albino female rats with an average weight of (200–220 g) were randomly divided  into 5 groups: Group I: (control group) rats were fed the standard diet, Group II: healthy rats that received Okra Powder (200 mg/kg) for 4 weeks; Group III (HFD/STZ group): Rats were fed with high-fat diet (HFD) (60% fat) for 4 weeks  and then injected low dose of STZ (35 mg/kg), Group IV:  Diabetic rats that received Okra Powder (200 mg/kg) for 4 weeks. GroupV: Diabetic rats that received metformin (200 mg/kg) for 4 weeks. At the end of experiment, biochemical parameters were measured. Liver samples were removed and 5-6 µ sections were made and stained by H&E and Sudan black staining.Results: The results showed that all the biochemical parameters, except HDL-C and serum insulin were increased in diabetic rats, while they were decreased in Okra supplementation group compared  to diabetic rats (p0.05). The liver structure alterations were improved in treated diabetic rats with Okra Powder and metformin. Conclusion: Our findings confirmed the potential anti-hyperglycemic and hypolipidemic effects of Okra Powder. Thus, it seems it has an important role in the management of type 2 diabete.