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Morphotypes and pigment profiles of halophilic bacteria: Practical data useful for novelty, taxonomic categorization and for describing novel species or new taxa

机译:嗜盐细菌的形态和色素特征:实用数据可用于新颖性,分类学分类和描述新物种或新分类群

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Halophilic bacteria were isolated from oil spill samples collected from West-coast of Goa. Bacteria were isolated from oil studded soil, salt marsh and offshore samples (A, A7, CSM, CB and CM) collected along the West coastline in Goa (India) i.e. Arambol beach, Calanguate beach, Candolim beach and Colva beach on Zobell Marine agar, R2A agar, Mannitol salt agar and Blood agar at temperature 22 to 24?°C. Isolates showed growth in the presence of hydrocarbons (1% phenanthrene and 2% bitumen). Diverse profiles of pigments were observed on different nutrient medium. Color of pigments produced on agar media recorded as per standard color chart. All isolates showed different growth pattern. Isolate no 11 (GOACSMMS-11) showed three different morphological features/growth patterns on Zobell Marine Agar and R2A medium in the presence of hydrocarbons. Results obtained yield new information which gives a clear idea about morphological features and pigmented profiles of hydrocarbon resistant morphotypes in the presence different media compositions. The presented datasets will be useful for studies on bacterial species showing high sequence similarity. Hence, generated data serves as a benchmark for to distinguish between genetically similar bacteria and for further research in phenotype based microbial diversity, microbial ecology of microorganisms and microbial systematics and taxonomy in addition to genotype data.
机译:从果阿西海岸收集的溢油样本中分离出了嗜盐细菌。细菌是从印度果阿沿西海岸线采集的油污土壤,盐沼和近海样本(A,A7,CSM,CB和CM)中分离出来的,这些样本是在Zobell Marine Agar上的Arambol海滩,Calanguate海滩,Candolim海滩和Colva海滩,R2A琼脂,甘露醇盐琼脂和血琼脂,温度为22至24℃。分离株在碳氢化合物(1%菲和2%沥青)存在下生长。在不同的营养培养基上观察到颜料的不同分布。琼脂培养基上产生的颜料的颜色按照标准比色表记录。所有分离株均表现出不同的生长方式。 11号分离株(GOACSMMS-11)在存在碳氢化合物的情况下在Zobell Marine Agar和R2A培养基上显示出三种不同的形态特征/生长模式。获得的结果产生了新的信息,该信息给出了在存在不同介质组成的情况下烃抗性形态型的形态特征和色素分布的清晰思路。提出的数据集将对显示高序列相似性的细菌种类的研究有用。因此,除基因型数据外,生成的数据可作为区分遗传相似细菌的基准,并可用于基于表型的微生物多样性,微生物的微生物生态学,微生物系统学和分类学的进一步研究。

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