Stability of Verticillium dahliae resistance in tissue culture-derived strawberry somaclones

摘要

Somaclonal variation is a novel source of useful traits in strawberry breeding. In our previous work we selected K40 clone from in vitro cultures of strawberry cv. Elsanta, which was characterized by high resistance to Verticillium dahliae. The objective of this work was to evaluate the stability and transmission of this trait during in vitro shoot proliferation and traditional clonal propagation from runners. K40, Elsanta and Senga Sengana plants were propagated in vitro for 45 generations/passages or for 4 generation of clonal propagation from runners. The resistance/susceptibility of such derived plants to V. dahliae was tested in controlled conditions in a greenhouse. Irrespective of the method of propagation, the plants of K40 clone were much more resistant to V. dahliae than the plants of the original cultivar and the plants of ‘Senga Sengana’, which is a cultivar considered as resistant to Verticillium wilt in field conditions. This proves that this trait elicited at somaclonal variant is stable and transferable both during in vitro micropropagation and clonal plant propagation from runners.