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Transcriptomic analysis reveals numerous diverse protein kinases and transcription factors involved in desiccation tolerance in the resurrection plant Myrothamnus flabellifolia

机译:转录组学分析揭示了复活植物 Myrothamnus flabellifolia 的干燥耐性中涉及的多种蛋白激酶和转录因子

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The woody resurrection plant Myrothamnus flabellifolia has remarkable tolerance to desiccation. Pyro-sequencing technology permitted us to analyze the transcriptome of M. flabellifolia during both dehydration and rehydration. We identified a total of 8287 and 8542 differentially transcribed genes during dehydration and rehydration treatments respectively. Approximately 295 transcription factors (TFs) and 484 protein kinases (PKs) were up- or down-regulated in response to desiccation stress. Among these, the transcript levels of 53 TFs and 91 PKs increased rapidly and peaked early during dehydration. These regulators transduce signal cascades of molecular pathways, including the up-regulation of ABA-dependent and independent drought stress pathways and the activation of protective mechanisms for coping with oxidative damage. Antioxidant systems are up-regulated, and the photosynthetic system is modified to reduce ROS generation. Secondary metabolism may participate in the desiccation tolerance of M. flabellifolia as indicated by increases in transcript abundance of genes involved in isopentenyl diphosphate biosynthesis. Up-regulation of genes encoding late embryogenesis abundant proteins and sucrose phosphate synthase is also associated with increased tolerance to desiccation. During rehydration, the transcriptome is also enriched in transcripts of genes encoding TFs and PKs, as well as genes involved in photosynthesis, and protein synthesis. The data reported here contribute comprehensive insights into the molecular mechanisms of desiccation tolerance in M. flabellifolia .
机译:木本复活植物Myrothamnus flabellifolia对干燥具有显着的耐受性。热解测序技术使我们能够分析脱水和补液过程中的红叶念珠菌的转录组。我们分别在脱水和补液过程中鉴定出总共8287和8542个差异转录基因。响应于脱水胁迫,上调或下调了大约295个转录因子(TFs)和484个蛋白激酶(PKs)。其中,转录本水平的53 TFs和91 PKs迅速增加,并在脱水过程中达到顶峰。这些调节剂转导分子途径的信号级联反应,包括上调ABA依赖和独立的干旱胁迫途径以及激活应对氧化损伤的保护机制。上调抗氧化剂系统,并修改光合作用系统以减少ROS的产生。次级代谢可能参与了叶小叶藻的脱水耐性,这是由参与异戊烯基二磷酸生物合成的基因的转录本丰度增加所表明的。编码晚期胚发生丰富蛋白和蔗糖磷酸合酶的基因的上调也与对脱水的耐受性增加有关。在补液过程中,转录组还富含编码TF和PK的基因的转录本,以及参与光合作用和蛋白质合成的基因。此处报道的数据有助于深入了解f.lilabellifolia脱水干燥的分子机制。

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