Evaluation of resistance in a selected field strain of Haemonchus contortus to ivermectin and moxidectin using the Larval Migration on Agar Test

摘要

> face="Verdana, Arial, Helvetica, sans-serif" size="2">Haemonchus contortus is one of the most common and economically significant causes of disease in small ruminants worldwide, and the control programs of parasitic nematodes - including H. contortus - rely mostly on the use of anthelmintic drugs. The consequence of the use of this, as the sole sanitary strategy to avoid parasite infections, was the reduction of the efficacy of all chemotherapeutic products with a heavy selection for resistance. The widespread of anthelmintic resistance and the difficulty of its early diagnosis has been a major concern for the sustainable parasite management on farms. The objective of this research was to determine and compare the ivermectin (IVM) and moxidectin (MOX) effect in a selected field strain of H. contortus with a known resistance status, using the in vitro larval migration on agar test (LMAT). Third stage larvae of the selected isolate were obtained from faecal cultures of experimentally infected sheep and incubated in eleven increasing diluted concentrations of IVM and MOX (6, 12, 24, 48, 96, 192, 384, 768, 1536, 3072 and 6144 size="2">µ face="Verdana, Arial, Helvetica, sans-serif" size="2">g/mL). The dose-response sigmoidal curves were obtained using the R2 value of 0.90 and the lethal concentration (LC₅₀) dose for the tested anthelmintic drugs using a four-parameter logistic model. The LC₅₀ value for MOX was significantly lower than IVM (1.253 size="2">µ face="Verdana, Arial, Helvetica, sans-serif" size="2">g/mL and 91.06 size="2">µ face="Verdana, Arial, Helvetica, sans-serif" size="2">g/mL), identifying the H. contortus isolate as considerably less susceptible to IVM compared to MOX. Furthermore, the LMAT showed a high consistency (p0.0001) and provided to be a useful diagnostic tool for monitoring the resistance status of IVM and MOX in H. contortus field isolate, as well as it may be used for official routine drug monitoring programs under the Ministry of Agriculture (MAPA) guidance.