声明
英文缩略表
Chapter 1 Introduction
1.1 Parasitic infections
1.2 The helminths
1.3 Control of helminths
1.4 Piperazine
1.5 Benzimidazoles
1.6 Imidazothiazole, and tetrahydropyrimidines
1.7 Macrocyclic lactones
1.8 IVM resistance
1.9Haemonchus contortus
1.10 Problem
1.11 Solution
Chapter 2Analysis of the Background Genetic Variation of Various Populations ofHaemonchus contortusfrom Field, Laboratory, and Resistant StrainsUsing Genome-Wide Data
2.1 Abstract
2.2 Introduction
2.3 Methods
2.3.1Collection of worms
2.3.2DNA extraction, library construction and pool sequencing
2.3.3SNP calling
2.3.4Characterization of genetic variability
2.3.5Selective sweep analysis
2.3.6 Statistical analysis
2.4 Results
2.4.1SNP description and characterization
2.4.2Nucleotide diversity and population differentiation
2.4.3Identification of selective sweeps
2.4.4Functional annotation of private selective sweeps
2.5 Discussion
2.6 Conclusions
Chapter 3 Microsatellite Analysis Reveals Extensive Gene Flow, and Lack of Population Structure in the Farm Populations of
3.1 Abstract
3.2 Introduction
3.3 Methods
3.3.1Worm collection, identification, and DNA extraction
3.3.2Microsatellite genotyping
3.3.3Data analysis
3.4 Results
3.4.1Allele frequency analysis
3.4.2Genetic diversity and Hardy-Weinberg equilibrium
3.4.3Genetic differentiation, population structure and clustering
3.5 Discussion
3.6 Conclusions
Chapter4 Whole Genome Re-Sequencing Reveals the Candidate Genes Associated with Ivermectin Resistance inHaemonchus
4.1 Abstract
4.2 Introduction
4.3 Methods
4.3.1Background of strains, worm collection and identification
4.3.2DNA isolation, library construction, and whole genome re-sequencing
4.3.3Mapping reads, and variant calling
4.3.4Characterization of genetic variability /and tracing selection
4.4 Results
4.4.1 Results of whole genome re-sequencing
4.4.2 Results of population genomics analyses
4.5 Discussion
4.6 Conclusions
Chapter5 Functional Validation of Candidate Genes Associated with Ivermectin Resistance inHaemonchus Contortusby RNAi Based Gene Silencing
5.1 Abstract
5.2 Introduction
5.3 Methods
5.3.1Egg extraction
5.3.2Culturing eggs into larvae
5.3.3RNAi based silencing of candidate genes
5.3.4Larval feeding inhibition assay (LFIA)
5.3.5RNA extraction and qPCR
5.3.6 Statistical analysis
5.4 Results
5.4.1Results of egg extraction and larvae culture
5.4.2Results of siRNA uptake
5.4.3Results of RNAi and LFI assays
5.5 Discussion
5.6 Conclusions
Conclusions
参考文献
致谢
Curriculum Vitae
中国农业科学院;
