Expression profile of the SOX9 gene in the testes of sexually immature and mature male goats (Capra hircus), and its potential influence on postnatal testis development

摘要

The aim of this study was to compare the expression levels of the SOX9 (SRY-box 9) gene in the testesof 18 White improved male goats (Capra hircus) divided into three age groups (one, 10 and 15 months of age withseven, eight and three individuals per group, respectively). Abnormalities in testis development were observed inthree individuals from the group of 10-month-olds. Additionally, differences in SOX9 expression that may affectthe process of testis maturation and testicular spermatogenic activity were investigated among individuals. Theexpression of the SOX9 gene in testicular tissues was analysed using qPCR. Maximal SOX9 expression was observedin 10-month-old males with normal testes, while expression was significantly reduced in the same age group ofmales with abnormal testes. Abnormalities in testis development were associated with low parameters of semenquality. The lowest expression levels of SOX9 were observed in 15-month-old goats. The results of the presentstudy indicate that SOX9 expression changes significantly during the development of male goats.