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首页> 外文期刊>Turkish Journal of Fisheries & Aquatic Sciences >Genetic Divergence between Auxis thazard and A. rochei based on PCRRFLP Analysis of mtDNA D-loop Region
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Genetic Divergence between Auxis thazard and A. rochei based on PCRRFLP Analysis of mtDNA D-loop Region

机译:基于mtDNA D-loop区PCRRFLP分析的无锡拟南芥与罗氏拟南芥的遗传差异

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摘要

Tunas of genus Auxis are commercially and ecologically important fish of family Scombridae. There is currently no information concerning the genetic divergence between A. thazard and A. rochei. Therefore, a study was conducted to estimate the degree of genetic divergence between two species based on PCR-RFLP analysis of D-loop region (500 bp) of mitochondrial DNA in 100 individuals collected from Tuticorin (India). High level of genetic divergence (9.26%) was observed between A. thazard and A. rochei. Estimates of molecular diversity indices were higher in A. rochei (π = 3.8808; hd = 0.9641) compared to A. thazard (π = 2.2514; hd = 0.8441). Based on demographic parameters, population expansion of A. thazard estimated to begin about 67,167 years before present while of founding of A. rochei took place about 114,747 years before present in Indian Ocean. Thus, we conclude that A. rochei represents the ancestral species of genus Auxis.
机译:Auxis属的Tunas是Scombridae科的商业上和生态上重要的鱼类。目前尚无关于tazar和罗氏曲霉之间遗传差异的信息。因此,进行了一项研究,以对从图蒂卡林(印度)收集的100名个体中线粒体DNA的D环区域(500 bp)的PCR-RFLP分析为基础,估算了两个物种之间的遗传差异程度。在t。Ahazard和罗氏曲霉之间观察到高水平的遗传差异(9.26%)。罗氏曲霉(π= 3.8808; hd = 0.9641)的分子多样性指数估计值高于危险曲霉(π= 2.2514; hd = 0.8441)。根据人口统计参数,t。tazar的种群扩展估计开始于目前的67,167年,而rochei的建立则发生于印度洋的大约114,747年。因此,我们得出结论,罗氏曲霉代表了Auxis的祖先种。

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