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首页> 外文期刊>Turkish journal of chemistry >A novel glucose chemiluminescence biosensor based on a rhodanine derivative chemiluminescence system and multilayer-enzyme membrane
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A novel glucose chemiluminescence biosensor based on a rhodanine derivative chemiluminescence system and multilayer-enzyme membrane

机译:基于罗丹宁衍生物化学发光系统和多层酶膜的新型葡萄糖化学发光生物传感器

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摘要

Using glucose oxidase as a model enzyme, a novel rhodaninederivative chemiluminescence biosensor for the determination ofglucose was formed based on multilayer-enzyme membrane as receptor,which was assembled via layer-by-layer assembly of sol-gel and glucoseoxidase-gold nano-particles inside a glass tube. Compared with thetraditional chemiluminescence biosensor, the proposed biosensor hadsome remarkable advantages, such as good selectivity of substrate,good response performance, good stability, good sensitivity, andlonger service life. The linear range was from 1.0 imes 10^{-6} to5.0 imes 10^{-2} mol L^{-1} (r = 0.9991). The detection limit was5.0 imes 10^{-7} mol L^{-1}. In this work, the optimum number oflayers was 4, and the Michaelis-Menten constant of immobilized glucoseoxidase was 0.2 mmol L^{-1}. The proposed method was applied to thedetermination of glucose in 6 different human serum samples withsatisfactory results.
机译:以葡萄糖氧化酶为模型酶,以多层酶膜为受体,构建了新颖的用于检测葡萄糖的若丹宁衍生物化学发光生物传感器。玻璃管。与传统的化学发光生物传感器相比,该生物传感器具有底物选择性好,响应性能好,稳定性好,灵敏度高,使用寿命长等显着优点。线性范围是1.0×10 ^ {-6}至5.0×10 ^ {-2} mol L ^ {-1}(r = 0.9991)。检出限为5.0×10 ^ {-7} mol L ^ {-1}。在这项工作中,最佳层数为4,固定化葡萄糖氧化酶的Michaelis-Menten常数为0.2 mmol L ^ {-1}。该方法用于6种不同人血清中葡萄糖的测定,结果令人满意。

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