A novel glucose chemiluminescence biosensor based on a rhodanine derivative chemiluminescence system and multilayer-enzyme membrane

摘要

Using glucose oxidase as a model enzyme, a novel rhodaninederivative chemiluminescence biosensor for the determination ofglucose was formed based on multilayer-enzyme membrane as receptor,which was assembled via layer-by-layer assembly of sol-gel and glucoseoxidase-gold nano-particles inside a glass tube. Compared with thetraditional chemiluminescence biosensor, the proposed biosensor hadsome remarkable advantages, such as good selectivity of substrate,good response performance, good stability, good sensitivity, andlonger service life. The linear range was from 1.0 imes 10^{-6} to5.0 imes 10^{-2} mol L^{-1} (r = 0.9991). The detection limit was5.0 imes 10^{-7} mol L^{-1}. In this work, the optimum number oflayers was 4, and the Michaelis-Menten constant of immobilized glucoseoxidase was 0.2 mmol L^{-1}. The proposed method was applied to thedetermination of glucose in 6 different human serum samples withsatisfactory results.