Immunoproteomic analysis of Trichinella spiralis larval crude antigens recognized by sera from patients with trichinellosis after treatment with albendazole

摘要

Antibody responses and antigen recognition were monitored during and aftertreatment with albendazole (ABZ) in nine patients selected from a trichinellosis outbreak thatoccurred in north-west Poland in 2007. Seven out of the nine patients yielded positive serumIgG response during treatment. One month after treatment, the IgG response decreased inmost patients. Serum levels of ABZ and main metabolites greatly varied among patientswithout correlation with the IgG response. Two-dimensional electrophoresis and westernblot with serum from each patient showed highly immunoreactive spots located between 3–10 pI and 45–97 kDa in all patients. Matrix-assisted laser desorption ionization–time-of-flightmass spectrometry (MALDI–TOF) and MALDI–TOF/time-of-flight mass spectrometry (TOFMS) analysis identified actine, enolase, p49 protein, Caenorhabditis elegans-targeted antigen,and serine protease as the most reactive proteins. A minor spot located at 6 pI and 26 kDaidentified as annexin I failed recognition in most patients showing decline in IgG responseand clinical improvement after treatment. This protein could constitute a sensitive markerfor the effectiveness of ABZ against trichinellosis.