Cardioprotective effect of salvianolic acid B against isoproterenol-induced inflammation and histological changes in a cardiotoxicity rat model

摘要

Purpose: To investigate the cardioprotective role of salvianolic acid B (SAB-B) against isoproterenol (ISO)-induced cardiotoxicity in a rat model. Methods: A total of 32 male rats were equally divided into four groups as follows: control group given saline only; rats treated with ISO (85 mg/kg; i.p.) for 2 consecutive days (MI model; ISO group); rats pre-treated with SAB-B (20 mg/kg, i.p.) prior to exposure to ISO (SAB-B+ISO, treatment group), and rats administrated SAB-B (20 mg/kg, i.p.) for 28 days (SAB-B group). Results: Pre-treatment with SAB-B for 28 days enhanced the levels of various antioxidants (CAT, SOD and GSH) and hemodynamic parameters, viz, systolic blood pressure (SBP), diastolic blood pressure (DBP) and mean arterial pressure (MAP). Likewise, substantial decrease in the levels of lipid peroxidation product (MDA), inflammatory markers (NF-κb, TNF-α, IL-1β and IL-6), heart weight, heart-to-body weight ratio, and serum cardiac markers (CPK and LDH) were seen in SAB-B treated rats, relative to ISO-treated group. Rats in SAB-B group had significantly downregulated protein ex pressions of NF-κb and TNF-α, and significant reduction in histopathological changes (edema and necrosis) after 28 days of treatment. Conclusion: SAB-B possesses potent cardio-protective potential against ISO-induced cardiotoxicity due to its antioxidant, anti-inflammatory and anti-necrotic properties.