MiR-145 inhibits proliferation of primary colon adenocarcinoma cells via induction of apoptosis, cell cycle arrest and inhibition of cell migration

摘要

Purpose: To investigate the role and potential of miR-145 as a therapeutic target for the treatment of primary colon adenocarcinoma in cell lines Methods: The ex pression of miR145 was determined by quantitative real time-polymerase chain reaction (RT-PCR), while cell viability was determined by MTT assay. Apoptosis was assessed by 4',6-diamidino-2-phenylindole (DAPI), acridine orange/ethidium bromide (AO/EB), and annexin V/PI double staining. Cell cycle analysis was performed by flow cytometry, while immunoblotting was used to determine protein ex pression. Results: The ex pression of miR-145 was significantly enhanced in all the colon adenocarcinoma cell lines investigated. On the other hand, suppression of miR-145 ex pression led to significant decrease in cell viability, activation of apoptosis, G2/M cell cycle arrest, and inhibition of migration of colon adenocarcinoma cells. Conclusion: These results indicate that miR-145 regulates the proliferation and metastasis of colon adenocarcinoma cells. Thus, it may be a prospective drug target for the treatment of this disease.