Mangiferin ameliorates hyperglycemia by inhibiting oxidation and α-glucosidase activity

摘要

Objective Mangiferin (MF) is a polyphenol isolated from the root of AnemarrhenaasphodeloidesBge.. This study was aimed to investigate the effects of MF on hyperglycemia in animal models of insulin resistance and streptozotocin (STZ)-induced diabetes. Methods The diabetes mellitus model was established in mice by receiving a multiple hypodermic injection of hydrocortisone sodium succinate (HCSS) (70 mg/kg) or a single intravenous injection of STZ (130 mg/kg). Meanwhile MF at different dosage (50, 100 and 200 mg/kg) were oral administrated for consecutive 10 days. Data of blood glucose were collected at different time after intraperitoneal injection of insulin (0.5 U/kg) to investigate the insulin resistant. As well as the oxygen radical absorbance capacity (ORAC) and superoxide dismutase (SOD) activity of kidney were measured. The in vitro experiment was established to investigate the inhibitory capacity of MF to α-glucosidase.Results Oral administration of MF significantly prevented insulin resistance caused by HCSS injection. STZ-induced diabetic symptoms were also improved, including fasting blood glucose, glycated hemoglobin, plasma triglycerides, hepatic glycogen, kidney SOD and ORAC level. The in vitro experiment demonstrated that MF had potent α-glucosidase inhibitory activity. Conclusion The obtained results demonstrate that MF ameliorates insulin resistance and STZ-induced glucose metabolism disturbance. The MF exerts the protective effects through improving the antioxidant ability, promoting hepatic glycogen synthesis and inhibiting α-glucosidase activity. Highlights Mangiferin (MF), a polyphenol extracted from the root of AnemarrhenaasphodeloidesBge., significantly ameliorates insulin resistance and streptozotocin (STZ)-induced diabetic symptoms. Potential mechanisms were involved in improving the antioxidant ability and inhibiting α-glucosidase activity. This study provides corresponding evidence for the clinical application of mangiferin.