Research on Xijiao Dihuang Decoction suppressing platelet apoptosis in immune-mediated aplastic anemia based on mitochondrial mediated pathway

摘要

Highlights Excessive platelet apoptosis is one of the pathogenic cause for immune-induced aplastic anemia. In the present study, we established an immune-induced aplastic anemia model and gave Xijiao Dihuang Decoction lavage daily. The results of this study indicated Xijiao Dihuang Decoction could increase platelet number and prevent its apoptosis through the mitochondrial pathway. The aplastic anemia mice model was established in this study. BALB/c mice shall be treated with whole body irradiation with 60 Co-γradiation (5.5Gy, 1.1Gy/min × 5 min). then within 4 h, DBA/2 mice were injected lymphocyte suspension 1 × 106 cells /mouse through caudal vein. Grouping of testing animals Normal control mice were healthy C57BL/6 mice without AA modeling. AA control group mice were exposed to radiation and cell transfusion and had no treatment with either CSA or XDD. CSA group mice received daily lavage with 0.027g/kg (0.1ml/10g) of CSA whereas XDD group mice received daily lavage with 19.5g/kg of XDD. The experimental result indicated CSA and XDD lavage mice had significantly higher platelet count and ΔΨm than AA mice (P 2+ were significantly lower in CSA and XDD groups compare with AA group. More specifically, compared to CSA group, XDD group also had lower level of ΔΨm and higher level of Cyt C and Ca2+ (both P P P 0.05). Therefore, we hypothesized XDD was possible to increase platelet number and prevent its apoptosis in immune-induced AA via the mitochondrial pathway.