LAGENARIA SICERARIA AS AMELIORATIVE AGENT AGAINST INFLAMMATION AND FIBROSIS INDUCED BY SODIUM ARSENITE ON THE HISTOMORPHOLOGY OF RAT LIVER.

摘要

Objectives: To observe the effect of lagenaria siceraria on inflammation andfibrosis brought about by arsenic in liver of Sprague Dawley rat. Study Design: Laboratorybased randomized control trial. Place and Duration of Study: This experiment was performed atDepartment of Anatomy, Army Medical College Rawalpindi in co-operation with National Instituteof Health (NIH) Islamabad for eight weeks (1st March 2017 to 25th April 2017). Material andMethods: Fifty Sprague Dawley rats (both male and females housed separately) were carefullychosen and distributed randomly into five groups, each consisting of 10 animals. A and B werethe control groups whereas C, D and E served as experimental groups. During the first fourweeks, experiment groups C, D and E were given a dosage of 5milligram/kilogram body weightof sodium arsenite. At the end of four weeks, animals from control group A and experimentalgroup C were dissected and liver samples were processed for microscopic studies. In the next4 weeks, group D animals were set aside without any further intervention. At that time, sodiumarsenite at a dose of 5 milligram/kilogram body weight and lagenaria siceraria at a dose of 100milligram/kilogram bodyweight were administered to group E animals. Group B animals servedas control for experimental groups D and E. At the end of these 4 weeks animals of groups B, Dand E were dissected. Results: Experimental group C developed moderate grade fibrosis andinflammation (grade 2 to 3) as compared to group A. Degree of inflammation and fibrosis wasmild to moderate (grade 1 to 2) in group D. There was no inflammation and fibrosis (Grade 0 to1) in group E. Group B served as a control for group D and E. Conclusion: Inflammation andfibrosis developed in the liver of adult rats when they were subjected to sodium arsenite evenfor a brief calculated period. Simultaneous administration of lagenaria siceraria can shield anddiminish the toxic effects of arsenic. Oxidative potential and immunomodulatory properties andpresence of flavonoid like substances renders lagenaria siceraria to act as ameliorative againstthis fibrosis and inflammation in liver lobules and surrounding area of portal triads.