Chemical Constituents and Cytotoxicity Activity of Phaleria Macrocarpa (SCHEFF) Boerl. Fruits on MCF-7 Cell Line

摘要

Successive extraction method of dried fruits of Phaleria macrocarpa had afforded chloroform, ethyl acetate and ethanol crudeextracts. Two benzophenone derivatives which are 2,4,4'-trihydroxy-6-methoxybenzophenone (1) and 4,4'-dihydroxy-2-methoxy- benzophenone-6-O-β-D-glucopyranoside (2) were isolated from the ethyl acetate crude extract. The structures ofthese compounds were elucidated by spectroscopic techniques using Infrared spectroscopy (IR), 1D (1H, 13C, DEPT), 2D(COSY, HMQC, HMBC) NMR spectroscopy and mass spectrometry. The cytotoxic activity of the crude extracts andbenzophenones were tested against human breast carcinoma cell line (MCF-7). MTT assay method was applied to measure theviability of cells at eight different concentrations. The ethyl acetate crude extract was showed higher cytotoxicity activity withIC50 value 126.67 μg/mL compare with ethanol extract (218.33 μg/mL) and chloroform extract (278.33 μg/mL). Meanwhile,benzophenone (1) and benzophenone (2) were exhibited potent cytotoxic properties with IC50 values 141.00 μg/mL and 166.00μg/mL, respectively.