Listeriolysin O, but not Murine E-cadherin, is Involved in Invasion of Listeria monocytogenes into Murine Liver Parenchymal Cells

摘要

Human E-cadherin and listeriolysin O (LLO) are involved in invasion of Listeria monocytogenes into humanliver parenchymal cells (LPC). Yet, it remains to be determined whether murine E-cadherin and LLO participate in invasionof L. monocytogenes into murine LPC. In the present study, involvement of murine E-cadherin and LLO in invasionof L. monocytogenes into murine LPC was investigated. Murine E-cadherin was expressed on murine LPC, but the expressionbecame undetectable by insertion of transgene of Simian virus 40 large T antigen. Although invasion of L. monocytogenes into murine LPC was found regardless of murine E-cadherin expression, infection rate of L. monocytogenes beingunable to secrete LLO was lower than that of L. monocytogenes being capable of secreting LLO. Our RESULTS verifythat invasion of L. monocytogenes into murine LPC occurs independently of murine E-cadherin and indicate that LLOparticipates in invasion of L. monocytogenes into murine LPC.