Thrombosis and cardiovascular-related diseases can be caused by fibrin aggregation. Thrombolytic agents have been used totreat thrombolytic problems and cardiovascular-related diseases. There is a need to search for new thrombolytic agents frombacteria, fungi, insects and fermented foods. To name a few, recently, fungi such as Aspergillus oryzae KSK-3, Fusarium sp.CPCC 480097 and Fusarium sp. BLB were found to be able of producing protease fibrinolytic enzyme. Potential proteasefibrinolytic enzymes were observed from Lignosus rhinocerus collected from the forest of Sg Perak. Out of the 6 sclerotiaobtained, one of them named LR-1 produced the maximum clear zone on Skim Milk Agar. LR-1 was then tested on fibrin plateincubated at 37°C for 18 hours and produced a clear zone as compared to positive control, plasmin. Leaves of Hibiscus sp. werewiped with 70% alcohol and 70% sodium hypochlorite for sterile disinfectant. The leaves were then cut into pieces of 4cm andplaced on Potato Dextrose Agar. Fungi grown from the leave pieces were sub cultured onto a new Potato Dextrose Agar.16endophytic fungi were isolated from Hibiscus sp. leaves from six locations in Shah Alam, Selangor. Four isolated endophyticfungi showed positive protease activity on Skim Milk Agar.
展开▼
