Cytotoxicity study of Nigella Sativa and Zingiber Zerumbet Extracts in Human Myeloid Leukemia Cell Lines (HL60)

摘要

The cytotoxicity effects of the Nigella sativa (Ns) and Zingiber zerumbet (Zz) plant extracts on acute Human myeloid leukemiacell lines (HL60) are reported. The HL60 cell lines were purchased from the American Tissue Culture Company (ATCC) to betreated with Petroleum ether (PE) and Aqueous (AQ) extracts of Ns and Zz extracts of Ethanol (ET) and Hexane (HEX). In anattempt to explain cytotoxicity effects of Ns and Zz extracts in these cell lines, in this experiment, we examined the effects ofDoxorubin (Dox) on their sensitivity as anticancer drugs which act as a positive control. Different plant species were found tohave different components of bioactive compounds. The different activities are represented by the presence of differentcompounds. It was observed that the cytotoxicity effects of plant species are differed based on the presence of the bioactivecompounds. Therefore, this study is carried out to compare the cytotoxicity effects of Ns and Zz extracts on HL60 cell lines. Inthis study, Hexane extract of Zz significantly drop off the percentage of cell viability of HL60 cell with the lowest IC50 value of45ug/ml. Hexane extract is more effective solvent compared to ethanol that gave IC50 value of 260ug/ml. Ns extract is lesseffective on HL60 cell lines that gives gave the highest IC50 value of 660ug/ml for PE and more than 1000ug/ml for AQ. It isconcluded that the cytotoxicity effects of Zz is more compared to Ns in this cell lines. HEX extract is more effective for the Zzextraction which gave the lowest IC50 compared to ET. In addition, PE extract acts as a better solvent for Ns extractioncompared to AQ solvent since it gave the lowest IC50 value. The mode of cell death was investigated through annexin-v flow cytometrymethod and it was resulted the way of cell death is through apoptosis