Isolation of 62 kDa Protein with Antioxidant Properties from NaturalHoney

摘要

It is strictly evident that honey contains antioxidant activity; however, constituents of honey responsible for its antioxidantactivity are still needed to be investigated. The aim of this study was to purify protein fraction on the basis of antioxidantactivity. Fourteen natural honey samples from North Africa and South Asia were evaluated for their antioxidant activities byemploying 1,1-Diphenyl-2-picrylhydrazyl (DPPH) radical assay. The scavenging activity of all honey samples ranged 18.7-32.6%. A 62 kDa protein was isolated from honey by gel filtration chromatography followed by reverse phase HPLC. Thisprotein showed significant radical scavenging activity (54.6 %). This indicated the possible role of this protein in theantioxidant and therapeutic properties of the natural honey.