Chemical Composition and Antioxidant Properties of Backhousia Citriodora Volatile Oil

摘要

Backhousia citriodora commonly known as lemon myrtle belongs to Myrtaceae family. This species is an evergreen tree whichcan grow up to 30 meters. Lemon myrtle is highly aromatic, having an exquisite flavour and aroma of a blend of lemongrass,lime and lemon. The leaf and flowers are used in tea blends and beverages, dairy, biscuits, breads, confectionery, pasta, syrups,liqueurs, flavoured oils, dipping and simmer sauces. In our study, the chemical composition and antioxidant properties of thelemon myrtle volatile oil obtained via hydro distillation were investigated. Examination by gas chromatography (GC) and gaschromatography/mass spectrometry (GC/MS) analysis showed that the volatile oil obtained from the leaves was made uppredominantly neral (39.57%) and geranial (52.43%). Linalool (0.37%), citronellal (0.15%), E-isocitral (2.47%), geraniol(0.66%), ethyl geranate (0.13%), caryophyllene (0.08%) and spathulenol (0.09%) were the other component present insignificant amounts. For the stem part, similarly the major components also are neral (36.76%) and geranial (56.30%). Otherminor components found in stem oil were 6-methyl-5-hepten-2-one (0.13%), linolool (0.24%), citronellal (0.09%), trans-paramentha-2,8-dien-1-ol (0.39%), E-isocitral (0.92%), (3Z)-hexenyl-(3Z)-hexenoate (0.22%), 8-hydroxy-neo-menthol (1.17%),(E)-α-Damascene 0.70%), spathulenol (0.17%) and globulol (0.14%). The obtained volatile oils were screen for the antioxidantproperties by using DPPH (1,2-diphenyl-2-picryl-hydrazyl) radical scavenging assay and xanthine/xanthine oxidase superoxidescavenging assay. Our study indicated both the lemon myrtle leaf and stem volatile oil showed high antioxidant properties.DPPH scavenging activity for both the leaf and stem parts are 83.72% and 84.46% respectively. As for the xanthine/ xanthineoxidase superoxide scavenging assay, both the lemon myrtle leaf and stem volatile oil showed high superoxide scavengingactivity, which are 83.72% and 84.46% respectively.