Effect of ganglioside GT1b on the in vitro maturation of porcine oocytes and embryonic development

摘要

Ganglioside is an acidic glycosphingolipid with sialic acids residues. This study was performed to investigate the effect and mechanism of ganglioside GT1b in porcine oocytes in the process of in vitro maturation (IVM) and preimplantation development. Metaphase II (MII) rates were significantly (P 2+ in oocytes treated with different concentrations of GT1b was measured. Intracellular Ca2+ was significantly (P 2+ in the process of oocyte maturation regardless of the cell cycle stage. Therefore, GT1b is thought to act on another mechanism that controls intracellular Ca2+.